Coding

Part:BBa_K4632005:Design

Designed by: Haolong Lai   Group: iGEM23_SCAU-China   (2023-10-09)


dapA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 64
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

E. coli strain WM3064 is a mutant strain with a dapA gene mutation, resulting in a nutritional deficiency phenotype for diaminopimelic acid (DAP). In our team, we have a plug-and-play, biologically safe device was developed based on the nutritional deficiency of diaminopimelic acid and low oxygen (or anaerobic) induction. You can see more design detail in BBa_K4632012 [1]

Source

The dapA gene fragment was obtained by PCR amplification from E. coli TOP10.

References

[1] Dehio C, Meyer M. Maintenance of broad-host-range incompatibility group P and group Q plasmids and transposition of Tn5 in Bartonella henselae following conjugal plasmid transfer from Escherichia coli[J]. J BACTERIOL, 1997,179(2):538-540.